• Luminescent ATP Cell Viability Assay Kit I Workflow

  2026-08-24

  Build faster cell viability measurement workflows for ferroptosis, proliferation, and drug-response studies with ATP-dependent luciferase luminescence detection. The kit combines a 10–30,000-cell linear range with a no-prelysis format, making it especially useful for resolving combination-treatment effects such as ciprofloxacin plus RSL3.

• Caspase-3 Fluorometric Assay Kit in RCC Apoptosis

  2026-08-24

  Use DEVD-AFC fluorescence to quantify executioner caspase activity in drug-treated renal cancer cells, then connect the signal to mitochondrial stress, ROS, and autophagy controls. This workflow turns a rapid apoptosis assay into a mechanistic experiment with practical normalization and troubleshooting guidance.

• 3-Bromopyruvate Reverses Cetuximab Resistance in CRC

  2026-08-23

  The 2023 Cancer Gene Therapy study shows that combining 3-bromopyruvate with cetuximab can suppress cetuximab-resistant colorectal cancer models through coordinated ferroptosis, autophagy, and apoptosis. Its mechanistic contribution is the identification of FOXO3a restoration and downstream AMPKα/pBeclin1 and PUMA signaling as a potential framework for studying resistance reversal.

• Septin4, VHL, and HIF-1α in Cardiomyocyte Apoptosis

  2026-08-22

  The reference study identifies HIF-1α as a previously unrecognized Septin4-interacting protein in hypoxia-exposed cardiomyocytes and shows that Septin4 strengthens VHL-dependent HIF-1α degradation. Its findings connect a mitochondrial proapoptotic protein to oxygen-sensing proteostasis, providing a mechanistic framework for studying hypoxia-inducible factor stabilization in cardiac injury models.

• Anlotinib Hydrochloride: From Target to Tumor Model

  2026-08-21

  Anlotinib hydrochloride is a multi-target tyrosine kinase inhibitor with a distinctive profile across receptor pharmacology, angiogenesis assays, and translational cancer research. This guide connects quantitative endothelial readouts with disease-model interpretation and highlights how a case report can improve experimental decisions.

• Pregnenolone Carbonitrile: Translational PXR Insight

  2026-08-20

  Pregnenolone Carbonitrile is more than a benchmark inducer of CYP3A. This thought-leadership analysis connects rodent PXR activation with gut–liver signaling, YAP-dependent hepatoprotection, hepatic stellate cell biology, and practical translational study design.

• ddhCTP: A Mechanism-First Guide to Antiviral Assays

  2026-08-20

  ddhCTP is a chain-terminating nucleotide analog that helps researchers distinguish direct RdRp inhibition from broader Viperin-mediated antiviral effects. This guide translates recent coronavirus findings into a mechanism-first framework for flavivirus studies, HEK293T assays, and antiviral drug development.

• Indometacin Sodium: From COX Biology to Translation

  2026-08-19

  A translational framework for using Indometacin Sodium Trihydrate in inflammation, pancreatic stellate cell, oligodendrocyte, and reproductive research while separating mechanistic opportunity from clinical and assay limitations.

• SIRT1, Mitochondrial Biogenesis, and Prion Toxicity

  2026-08-19

  The 2024 study identifies SIRT1-dependent PGC-1α–TFAM signaling as a mechanistic link between mitochondrial biogenesis and PrP106–126-induced neuronal injury in N2a cells. Its rescue experiments show that SIRT1 activation, including treatment with resveratrol, can reduce mitochondrial dysfunction and apoptosis in this cellular prion model while highlighting important limits on translation to disease therapy.

• EZ Cap™ Firefly Luciferase mRNA: Assay Workflows

  2026-08-18

  Build sensitive reporter experiments around Cap1-enabled translation, an optimized poly(A) tail, and a practical RNase-conscious workflow. This guide connects cell-based gene regulation assays with exploratory mRNA-LNP studies and in vivo bioluminescence imaging while emphasizing controls, normalization, and troubleshooting.

• nor-Binaltorphimine dihydrochloride in Circuit Assays

  2026-08-18

  Explore how nor-Binaltorphimine dihydrochloride can function as a causal probe of spinal κ-opioid receptor signaling. This article translates a brain-to-spinal mechanical allodynia study into practical assay-design principles for opioid receptor pharmacology and pain modulation research.

• Dorsomorphin (Compound C) for AMPK Workflows

  2026-08-17

  Dorsomorphin (Compound C) enables reversible, time-controlled interrogation of AMPK while also exposing BMP-dependent effects that can shape interpretation. This practical guide connects macrophage inflammation assays with hepatocyte metabolism, autophagy regulation, and SMAD phosphorylation workflows.

• Veratridine for Chamber-Specific Cardiac Assays

  2026-08-17

  Veratridine is a voltage-gated sodium channel opener that can turn chamber-specific cardiomyocyte identity into a functional electrophysiology challenge. This article connects recent right ventricular hPSC-cardiomyocyte methods with rigorous assay design, interpretation, and translational limits.

• H3K18 Lactylation Drives NOD2 in Bilirubin Injury

  2026-08-16

  Li et al. identify an immunometabolic and epigenetic pathway in which bilirubin-associated glycolysis increases H3K18 lactylation, activates NOD2 transcription, and promotes astrocyte pyroptosis. The study connects chromatin regulation with MAPK/NF-κB signaling in bilirubin encephalopathy, while also highlighting experimental limitations that must be addressed before translation.

• Caspase-3 Fluorometric Assay Kit for Ferroptosis

  2026-08-15

  Quantify executioner-caspase activity while investigating apoptosis–ferroptosis crosstalk, drug response, and neurodegenerative disease models. This workflow combines DEVD-AFC fluorescence with practical controls and troubleshooting guidance for more defensible caspase activity measurement.

8780 records 2/586 page Previous Next 12345 下5页 Last page